Pulse Sequences


New pulse sequences for Bruker Avance:
3D (H)N(CO-TOCSY-CO)NH to assign IDPs [1]
3D (HN)CO-TOCSY(CO)NH to assign IDPs [1]
2D NC for Arg Side Chains to determine protonation states [2]

by Yuichi Yoshimura and Frans Mulder


[1] Easy and unambiguous sequential assignments of intrinsically disordered proteins by correlating the backbone 15N or 13C’ chemical shifts of multiple contiguous residues in highly resolved 3D spectra. Yoshimura Y, Kulminskaya NV, Mulder FA. J Biomol NMR. 2015 Feb;61(2):109-21.
Link to Article

[2] Unambiguous Determination of Protein Arginine Ionization States in Solution by NMR Spectroscopy. Yoshimura Y, Oktaviani NA, Yonezawa K, Kamikubo H, Mulder FA. Angew Chem Int Ed Engl. 2017 Jan 2;56(1):239-242.
Link to Article


Click on the individual pulse sequences under the Downloads menu (top right) for an ASCII version of the experiment.